Eugenie, 30, posted the adorable snap on Instagram to mark Beatrice’s 32nd birthday on Saturday. The picture shows the sisters smiling as they pose for the selfie.
The stunning photo was taken the day before Beatrice’s royal wedding last month.
Eugenie also shared a cute throwback picture of her and Beatrice from when they were younger.
In the caption, she said: “Happy happy Birthday dear Beabea.
“Here we are on the eve of your wedding. Still rocking a scrunchie or hair accessory (since the 90s) and huge smiles on our faces.”
The post prompted a string of comments from royal fans.
One wrote: “Such a beautiful shot of two sisters, no make up, no nonsense just sibling love.”
A second commented: “Happy Birthday to Princess Bea! I love these sisters!”
A third said: “Happy Birthday Beatrice! You are a great sister Eugenie!!!!”
READ MORE: Princess Beatrice birthday: How will Princess Beatrice celebrate?
The Duchess of York posted: “Happy Happy Birthday My Dearest Beatrice.
“8.8.88 at 8.18pm was one of the greatest and most proud moments of my life.
“You are unique and simply one of the most special people on this planet. Your ever loving and devoted Mum.”
And the Royal Family shared a happy birthday message for Beatrice on Twitter and Instagram.
Beatrice wed millionaire property tycoon Edoardo Mapelli Mozzi last month in a low-key ceremony in Windsor.
The couple tied the knot at the Royal Chapel of All Saints at Royal Lodge.
The bride stunned in a vintage dress and tiara both borrowed from the Queen.
In an unusual move, the wedding was not announced in advance, the ceremony was held in private and pictures of the event were not immediately released.
In a statement, Buckingham Palace said: “The couple decided to hold a small private ceremony with their parents and siblings following the postponement of their wedding in May.
“Working within Government guidelines, the service was in keeping with the unique circumstances while enabling them to celebrate their wedding with their closest family.”
Beatrice and Mr Mapelli Mozzi, known as Edo, had planned to wed in May but they were forced to postpone their big day due to the coronavirus pandemic.
The couple got engaged last year following a whirlwind romance.